CD73 (NT5E, ecto 5' nucleotidase, E5NT, NTE) is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 serves as a costimulatory molecule in activating T cells. The enzyme is a dimer of 2 identical subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. CD73 can be used as a marker of lymphocyte differentiation. CD73 deficiency occurs in a variety of immunodeficiency diseases. Other forms of 5 prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.
Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.