Storage:Avoid exposure to sunlight,Keep dry Product description :Human VEGF R1 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF R1 (FLT1) in cell culture supernatant, serum and plasma (EDTA, citrate).
VEGF R1 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a biotin-conjugated VEGF R1 detection antibody then incubated at room temperature. Following washing, a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and washed. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of acidic stop solution. The density of yellow coloration is directly proportional to the amount of VEGF R1 captured on the plate.