This kit allows for the determination of MP-Ab concentrations in Bovine serum, cell culture supernates and other biological fluids Principle of the Bovine Mycoplasma Pneumoniae Antibody(MP-Ab) ELISA Kit:
The kit assay Bovine MP-Ab level in the sample, use Purified Bovine MP-Ab antibody to coat microtiter plate wells, make solid-phase antibody, then add MP-Ab to wells, Combined MP-Ab antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of Bovine MP-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve. Specimen requirements
1. extract as soon as possible after Specimen collection,and according to the relevant literature, and should be experiment as soon as possible after the extraction. If it can’t, specimen can be kept in -20 ℃ to preserve, Avoid repeated freeze-thaw cycles.
2. Can’t detect the sample which contain NaN3, because NaN3 inhibits HRP active. Storage: 2-8℃.