1.Standard, Sample diluent
2.Add Standard, Sample diluent, incubate for 30 min at 37℃.
3.Wash 5 time,Add HRP-Conjugate reagent, incubate for 30 min at 37℃.
4.Wash 5 times,Add Chromogen Solution A and B, incubate for 30 min at 37℃.
5.Add Stopp Solution
6. Read absorbance at 450nm within 15 min Assay range: 60pmol/L - 1600pmol/L Description About Bovine (PROG)progesterone Elisa Kit:
Bovine (PROG)progesterone Elisa Kit allows for the determination of PROG concentrations in Bovine serum, cell culture supernates and other biological fluids.
Bovine (PROG)progesterone Elisa Kit level in the sample, use Purified Bovine PROG antibody to coat microtiter plate wells, make solid-phase antibody, then add PROG to wells, Combined PROG antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Bovine PROG in the samples is then determined by comparing the O.D. of the samples to the standard curve. Storage: 2-8℃ Shelf Life: 6 months