Principle of the assay:
The kit assay Fish 17α,20βOH-PROG level in the sample， use Purified Fish 17α,20βOH-PROG antibody to coat microtiter plate wells, make solid-phase antibody, then add 17α,20βOH-PROG to wells, Combined 17α,20βOH-PROG which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of 17α,20βOH-PROG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Description About Fish 17α,20βOH-PROG ELISA Kit:
Fish 17α,20βOH-PROG ELISA Kit allows for the determination of 17α,20βOH-PROG concentrations in Fish serum, blood plasma, and other biological fluids. Storage and validity:
2．validity： six months.