Description About Human Mycoplasma ELISA Kit:
Human Mycoplasma ELISA Kit allows for the determination of mycoplasma in Human serum, and other biological fluids.
The kit assay Human mycoplasma level in the sample，use Purified Human mycoplasma antibody to coat microtiter plate wells, make solid-phase antibody, then add mycoplasma to wells, Combined With mycoplasma, after washing and removing non-combinative antigen and other components ,then Combined mycoplasma antibody which with HRP labeled become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,, TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
Compared with the CUTOFF value, according to this to judge mycoplasma exist in the sample or not. Storage: 6 month at 4 Centigrade Shelf Life: 6 months