Assay range: 0.8μg/L -20μg/L Description About Human Npt ELISA Kit:
Human Npt ELISA Kit allows for the determination of Npt concentrations in Human serum, cell culture supernates and other biological fluids.
Human Npt ELISA Kit level in the sample, use Purified Human Npt antibody to coat microtiter plate wells, make solid-phase antibody, then add Npt to wells, Combined Npt antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Npt in the samples is then determined by comparing the O.D. of the samples to the standard curve. Storage and validity:
2．validity： six months