Description About Human Superoxide Dismutase Elisa Kit:
Superoxide dismutase (SOD) can catalyze the superoxide anion to undergo the disproportionation to produce hydrogen peroxide (H2O2) and oxygen (O2), which are important antioxidant enzymes in the organism.
Human Superoxide Dismutase Elisa Kit is used double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To pre-coated human superoxide dismutase (SOD) capture antibody coated microwells, followed by the addition of specimens, standards, HRP-labeled detection antibody, after incubation and thorough washing. Coloring with the substrate TMB, TMB is converted to blue by the peroxidase catalysis and converted to the final yellow color under the action of an acid. The color depth is positively correlated with the level of superoxide dismutase (SOD) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm to calculate the sample concentration. Research Use Only:For Research Use Only. Not for use in diagnostic procedures.