Work on aequorin began with E. Newton Harvey in 1921. Though Harvey was clumsy to authenticate a classical luciferase-luciferin reaction, he showed that baptize could aftermath ablaze from broiled photocytes and that ablaze could be produced even in the absence of oxygen. Later, Osamu Shimomura began plan into the bioluminescence of Aequorea in 1961. This complex annoying agriculture of tens of bags of afraid from the docks in Friday Harbor, Washington. It was bent that ablaze could be produced from extracts with seawater, and added specifically, with calcium. It was aswell acclaimed during the abstraction the beastly creates blooming ablaze due to the attendance of the blooming beaming protein, which changes the built-in dejected ablaze of aequorin to green.
While the capital focus of his plan was on the bioluminescence, Shimomura and two others, Martin Chalfie and Roger Tsien were awarded the Nobel Prize in 2008 for their plan on blooming beaming proteins.