B7-H4, also known as B7x and B7S1, is a member of the B7 family of immune costimulatory proteins. Mature B7-H4 is a 50 kDa - 80 kDa glycosylated molecule with a 28 kDa protein core. Partial sensitivity to cleavage by PI-PLC suggests a possible GPI linkage of mouse B7-H4 to the cell membrane. The 230 aa extracellular region of B7-H4 contains one Ig-like V-set domain and one Ig-like C2-set domain which is followed by a hydrophobic C-terminal region. Within the ECD, mouse B7-H4 shares 90% and 99% aa sequence identity with human and rat B7-H4, respectively. It shares 21% - 29% aa sequence identity with B7-1, B7-2, B7-H1, B7-H2, B7-H3, and PD-L2.
Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.