Assay range: 30μmol/L -1000μmol/L Purpose:
This kit allows for the determination of FFA concentrations in Human serum, cell culture supernates and other biological fluids Principle of Human FFA ELISA Kit:
The kit assay Human FFA level in the sample,use Purified Human FFA antibody to coat microtiter plate wells, make solid-phase antibody, then add FFA to wells, Combined FFA antibody which With HRP labeled,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human FFA in the samples is then determined by comparing the O.D. of the samples to the standard curve.