Anti-CT DNA (culf thymus DNA)

Description:
Calf thymus DNA (CT-DNA) was purchased from Sigma (St Louis, MO) and treated with 100 mM methoxyamine (MX) or 360 mM sodium borohydride before being re-suspended in reagent water to reduce the background number of aldehydic lesions.
CT-DNA exposed to 100 ?M P45Q or F23Q in the presence or absence of NADPH was treated with 100 mM MX at 37°C for 2 h to eliminate the existing ADL. The DNA was then precipitated and washed with ethanol to remove residual MX, re-suspended in PBS and incubated at 70°C for 2 h. The resulting increase in aldehydic sites was attributed to the depurination/depyrimidation of unstable quinone base adducts, as determined by the ASB assay.

Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.