Anti-Clostridium perfringens type D

Description:
Clostridium perfringens type D is the major cause of a fatal enterotoxaemia also known as pulpy kidney disease. Epsilon is the principal toxin of this organism and is produced as an essentially inactive prototoxin which becomes activated by proteolytic enzymes in the gut. The diagnosis of enterotoxaemia is usually based on clinical signs and pathological findings but demonstration of toxins in the gut is the only confirmatory diagnosis. The most widely used method for toxin detection is the mouse protection test which is cumbersome, expensive and time consuming and, as the treatment of animals involved in the test is inhumane, alternative in vitro tests are required. A number of serological assays have been used, including radial immunodiffusion, reverse passive haemaglutination and counterimmuno-electrophoresis . Methods used for measuring epsilon toxin include 2 ELISA tests, both using a double antibody sandwich technique . This study describes a 4-layer sandwich ELISA for the detection of epsilon toxin.

Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.