GranzymeB ELISA kit

Storage Instruction:Store the kit at 4°C.
Description:
Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes (CTLs) and NK cells. These granules contain other proteins including perforin, a pore-forming protein. Upon binding of the CTL to a target cell, by CTL-receptor and antigen-presenting MHC molecules on the target cell, the contents of the granules are released into the intercellular space where perforin penetrates the cell membrane and forms transmembrane pores. Granzyme B enters the cytosol of the target cell through these pores and activates the intracellular cascade of caspases resulting in the death of the target cell. Granzyme A also enters through the pores and is capable of inducing apoptosis in the target cell but the molecular mechanism of the pathway is not known. Not all the granzymes enter into the target cell, some of the granzymes leak into the peripheral blood and other biological fluids. Detectable amounts of granzymes have been found in circulation in healthy volunteers. These soluble granzymes can now be measured by the K-ASSAY  Human Granzyme B ELISA.