P-Cadherin ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
The human P-Cadherin Kit is a sandwich ELISA. The capture antibody is a polyclonal human P-Cadherin antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known P-Cadherin concentration are added to these wells and allowed to complex with the bound P-Cadherin antibody. A biotinylated human P-Cadherin polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of P-Cadherin present in the samples. The purpose of this kit is the in-vitro quantitative determination of human P-Cadherin in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and