IL-12R ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
The biological functions of IL-12 are mediated through specific receptors on T and NK cells. To date, two IL-12 receptor subunits have been identified in mouse and humans. These receptor subunits are members of the cytokine receptor superfamily. Their cytoplasmic regions contain the box1 and box2 motifs that exist in other cytokine receptors, and they are related closely over their entire length to the " type" cytokine receptor glycoprotein 130 and the receptors for leukemia-inhibitory factor and G-CSF. Thus, the two IL-12 receptor subunits were designated IL-12R1 and IL-12R2. Analyses of IL-12 binding to these receptors demonstrated that, when expressed in COS-7 cells individually, human IL-12R1 and IL-12R2 bind IL-12 with low affinity; however, when the two subunits are coexpressed, they confer both high and low affinity binding of IL-12 and IL-12 responsiveness. In contrast to the human IL-12 receptors, IL-12R1 is the primary binding component in the mouse conferring both high and low affinity binding sites, whereas IL-12R2 only binds weakly to IL-12.