ANG-II ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
Angiotensin II increases blood pressure by stimulating the Gq protein in vascular smooth muscle cells . ACE inhibitor drugs are major drugs against hypertension.Other cleavage products of ACE, 7 or 9 amino acids long, are also known; they have differential affinity for angiotensin receptors, although their exact role is still unclear. 
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ang-II. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Ang-II. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ang-II, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of Ang-II in the samples is then determined by comparing the O.D. of the samples to the standard curve.