ACA IgG ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
This immunoassay kit allows for the in vitro semi-quantitative determination of mouse ACA (IgG) concentrations in cell culture supernates, serum, plasma and other biological fluids. 
The microtiter plate provided in this kit has been pre-coated with purified ACA (IgG) antigen. Samples are then added to the appropriate microtiter plate wells and incubated. Then add Horseradish Peroxidase (HRP)-conjugated anti-mouse IgG to each well and incubate. Finally, a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. Calculate the valence of ACA (IgG) in the samples.