ADV IgA ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
The Adenovirus IgA（ADV IgA）antibody test kit is based on the principle of the enzyme immunoassay (EIA). Adenovirus antigen is bound on the surface of the microtiter strips. Diluted patient serum or ready-touse standards are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized Adenovirus antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use antihuman-gA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of the IgA antibodies is directly proportional to the intensity of the color.