cHEV IgM ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
This HEV IgM kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of IgM-class antibodies to hepatitis E virus in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis E virus.
This kit is a two-steps incubation, solid phase antibody capture ELISA assay in which polystyrene microwell strips are pre-coated with antibodies directed to human immunoglobulin M proteins (anti-μ chain). The patient’s serum/plasma sample is added and during the first incubation step, any IgM-class antibodies will be captured in the wells. After washing out all the other components of the sample and in particular IgG-class antibodies, the specific HEV IgM captured on the solid phase is detected by the addition of recombinant HEV ORF2 antigens conjugated to horseradish peroxidase (HRP-Conjugate). During the second incubation, the HRP-conjugated antigens will specifically react only with HEV IgM antibodies and after washing to remove the unbound HRP-Conjugate, Chromogen solutions are added into the wells.