Granzyme-b ELISA Kit

Storage Instruction:Store the kit at 4°C.
Description:
The Granzyme B Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). A monoclonal antibody specific for Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations and unknowns are pipetted into these wells.
During the first incubation, the Granzyme B antigen and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated.
After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Granzyme B present in the samples